. . . . . . . . . .    ANM 2010     3rd International Conference on Advanced Nano Materials     12-15 September 2010 - Agadir, Morocco Back    Abstract ANMM354 NANOSTRUCTURED ACTIVE BIOMATERIALS FOR CLINICAL APPLICATIONS N. Benkirane-Jessel Institut National de la Santé et de la Recherche Médicale (INSERM), Unité 977, Faculté de Médecine, 11 rue Humann, 67085, Strasbourg Cedex, France . In recent years, considerable effort has been devoted to the design and controlled fabrication of structured materials with functional properties. The layer by layer buildup of polyelectrolyte multilayer films (PEM films) from oppositely charged polyelectrolytes offers new opportunities for the preparation of functionalized biomaterial coatings. This technique allows the preparation of supramolecular nano-architectures exhibiting specific properties in terms of control of cell activation and may also play a role in the development of local drug delivery systems. Peptides, proteins, drugs or DNA, chemically bound to polyelectrolytes or Cyclodextrins (CDs), adsorbed or embedded in PEM films, have been shown to retain their biological activities. Recently, we have demonstrated for the first time the sequential induction of nuclear and/or cytoplasmic expression products, mediated by β-cyclodextrin embedded in a PEM film. In recent times, tissue engineering has merged with stem cell technology with interest to develop new sources of transplantable material for injury or disease treatment. Eminently interesting, are bone and joint injuries disorders because of the low self-regenerating capacity of the matrix secreting cells. Recently, we have reported that embedded growth factors into a multilayered film (planar surface or as capsules) can drive stem cells to the cartilage or bone differentiation. We selected a model system made from layer by layer poly-ℓ-glutamic acid (PℓGA) and poly-ℓ-lysine (PℓL) films into which BMP-2 and TGFβ1 have been embedded. Our results demonstrate clearly that we are able to induce in vivo bone formation and osteogenesis in embryonic stem cells mediated by growth factors embedded into a nanostructured and multilayered capsules. . © nanoAC . . . . . . . . . .

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