. . . . . . . . . .    ANM 2010     3rd International Conference on Advanced Nano Materials     12-15 September 2010 - Agadir, Morocco Back    Abstract ANMM166 NOVEL CATIONIC BASED BOLAAMHIPHILES FOR EFFICIENT GENE DELIVERY Majad Khan and Yi Yan Yang Institute of Bioengineering and Nanotechnology, 31 Biopolis Way, The Nanos #04-01, Singapore 138669, Singapore . Synthetic gene delivery vectors, especially cationic polymers have attracted enormous attention in recent decades because of their ease of manufacture, targettability and scaling up. However, certain issues such as high cytotoxicity and low transfection efficiency problems have also hampered the advance of non-viral gene delivery. In this study, we designed and synthesized a family of cationic based bolaamphiles capable of mediating highly efficient gene transfection. Furthermore, some of the bolaamphilphiles prepared had amphoteric character. The resulting amphiphilic molecules were able to form nanoparticles with size around 150-200 nm. The bolaamphiles were able to condense DNA efficiently into nanoparticles of size around 200 nm with positive zeta potential about 25-30 mV. Luciferase expression level and percentage of GFP expressing cells induced by these amphiphiles was significantly higher than those mediated with 25 kDa polyethyleneimine by at least four folds at their optimal N/P ratio 10 on HEK293, HepG2, NIH3T3 and HeLa cells.  In vitro cytotoxicity testing reveals that polyplexes from this polymer were less cytotoxic than those of PEI. This family of bolaamphilies may be a promising gene delivery vectors for future gene therapy. . © nanoAC . . . . . . . . . .

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